137th IMEG Seminar
Co-hosting: Kumamoto University Global COE Program
g Cell Fate Regulation Research and Education Unit h
y Date z Tue 23 March 2010
y Time z 12:30 - 13:30
y Venue z Conference Room, Ground Floor
@@@ Institute of Molecular Embryology and Genetics (IMEG)
y Title z Highly efficient induction of human pluripotent stem cells by using a vector based on Sendai Virus, an RNA virus that does not integrate into the host genome
y Speaker z Noemi Fusaki, PH.D.
@@@Leader, Unit of Cell Therapy & Regenerative Medicine
@@@@@DNAVEC Corporation, PRESTO Researcher, JST
y Abstract z
Induced pluripotent stem cells (iPSCs) have been generated from somatic cells by introducing reprogramming factors. Integration of foreign genes into the host genome is a technical hurdle for the clinical application. Here, we show that Sendai virus (SeV), an RNA virus and carries no risk of altering host genome, is an efficient solution for generating saferiPSCs. Sendai-viral generated human iPSC expressed pluripotency genes, showed demethylation characteristic of reprogrammed cells. SeV-derived transgenes were decreased during cell division and viral-free iPS cells differentiated to mature cells of the three embryonic germ layers in vivo and in vitro including beating cardiomyocytes, neurons, and pancreatic cells. Our data demonstrated that highly-efficient, non-integrating SeV-based vector system provides a critical solution for reprogramming somatic cells and will accelerate the clinical application.
y Contact Information z Global COE Research Promotion Office Ikuko Yamaguchi (Ext. 5006)